Journal: The Journal of Molecular Diagnostics : JMD

Article Title: Multiplex Solid-Phase Melt Curve Analysis for the Point-of-Care Detection of HIV-1 Drug Resistance

doi: 10.1016/j.jmoldx.2019.02.005

Figure Lengend Snippet: Nucleic acid detection methodology. A: Synthesized or amplified fluorescently labeled DNA was added to the oligonucleotide microarray. B: Labeled DNA hybridizes to microarray probe sets each sharing sequences with the same flanking region and a central nucleotide triplet complementary to each of the reported codons at a drug resistance mutation (DRM) position. C: Multiplexed melt curve analysis was used to identify the most stable heterodimer and thereby detect the correct codon at the DRM position. RFU, relative fluorescence unit.

Article Snippet: With the use of the Oligonucleotide Modeling Platform Developers Edition 1.0 software package (DNA Software Inc., Ann Arbor, MI), simulations were performed on 81,126 sequences: six codons × the 13,521 distinct 203-bp sequences (containing the 5′ and 3′ 100-bp flanking codon 103) in the LMIC data set.

Techniques: Synthesized, Amplification, Labeling, Microarray, Mutagenesis, Fluorescence

Journal: The Journal of Molecular Diagnostics : JMD

Article Title: Multiplex Solid-Phase Melt Curve Analysis for the Point-of-Care Detection of HIV-1 Drug Resistance

doi: 10.1016/j.jmoldx.2019.02.005

Figure Lengend Snippet: Detection platform. The instrument prototype included an inverted fluorescence microscope and a custom stage to place, heat, and image the oligonucleotide microarray. This platform was the developer platform for the HYDRA-1K system in that it used a silicon slide rather than a complementary metal oxide semiconductor chip and therefore required an external light detector.9

Article Snippet: With the use of the Oligonucleotide Modeling Platform Developers Edition 1.0 software package (DNA Software Inc., Ann Arbor, MI), simulations were performed on 81,126 sequences: six codons × the 13,521 distinct 203-bp sequences (containing the 5′ and 3′ 100-bp flanking codon 103) in the LMIC data set.

Techniques: Fluorescence, Microscopy, Microarray